In-vitro Antifungal Activities of Kombucha Tea Culture Supernatant Combined with Voriconazole against Vulvovaginal Candidiasis Clinical Isolates

Objective: To investigate the antifungal activity of voriconazole, with and without Kombucha tea culture, against Candida strains isolated from vulvovaginal candidiasis. Material Methods: The study included 150 females, within child-bearing periods, complaining valvovaginal were isolated, identified by conventional microbiological methods; confirmed Viteck-2 System. sensitivity isolates to voriconazole was performed, via Disc diffusion method. Resistant then subjected minimum inhibitory concentrations (MIC) investigation alone, in combination a culture broth micro-dilution method ranging 0.0048 10 μg/ml. ability Kombucha, eradicate biofilms investigated using crystal violet absorbance assay. Results: Eighty-nine isolated. From these 60 showed variable resistance patterns (57 resistant, 3 had dose-dependent susceptability). significantly decreased MIC50 all 5 0.625 μg/ml (p-value<0.01); additionally, MIC90 reduced 1.25 (p-value =0.000). Voriconazole at concentration 0.156 succeeded eradicating formed 18 after adding supernatant versus zero when alone. Conclusion: Black cultures could be promising agents treatment